[How to use Bombyx Trap Database]
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Fluorescent Site epidermis midgut small gut
colon rectum fat body
silk gland dermal gland brain, corpa allata, nervus
simple eye cephalic part wing disc
salivary gland oenocyte malpighian tube
prothoracic gland testis, ovary, genital organ tracheole
spiracle other foregut
ganglion colleterial gland antenna
compound eyes
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Apparent by ordinary Developmental stages Egg Larva Spinning Prepupa Cocoon Pupa Adult Moth
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Apparent by fluorescence Developmental stages Egg Larva Spinning Prepupa Cocoon Pupa Adult Moth
Fluorescent region
Organ/Tissue by fluorescence Developmental stages Egg Larva Spinning Prepupa Cocoon Pupa Adult Moth
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    The latest update on Bombyx Trap DataBase (Release 2) is now available. New!

    Bombyx Trap DataBase Release 2: 28-Feb-2011
    - Data of AyFib-GAL4 enhancer trap lines and Gene Trap lines were stored in the database.

  1. Bombyx Trap DataBase
  2. Since the fine silkworm genomic sequence has been available by the integration of Japan and China WGS data (The International Silkworm Genome Consortium, 2008), the post-genome researches of silkworm are accelerating extensively. Transposon mutagenesis is very useful tool for gene function analysis in post-genome research. This database contains information on reporter expressions and positions of transposon vectors (mutators) in transposon insertion lines (enhancer trap lines and gene trap lines), and is integrated into silkworm genome database, KAIKObase (Shimomura et al., 2009).

  3. GAL4 enhancer trap and gene trap in silkworm, Bombyx mori
  4. In Release 1, we edited an information on BmA3-GAL4 enhancer trap lines using GAL4/UAS system. For more details of BmA3-GAL4 enhancer trap, please see Uchino et al. (2008). GAL4/UAS system is useful to regulate introduced genes for gene functional analyses and material productions. It is essential for functional studies to develop tissue-specific and developmental stage-specific GAL4 strains. So far, BmA3-0052-2 strain, which expresses GAL4 in midgut, was employed for positional clonings of Y gene (Sakudoh et al., 2007) and nsd-2 gene (Ito et al., 2008) successfully. We hope that this system would be applicable for gene disruption, too.
    In Release 2, we add the data of AyFib-GAL4 enhancer trap lines with the fibroin promoter of a wild silkmoth, Antheraea yamamai (Sezutsu et al., 2009, Uchino et al., in preparation). Also we add the data of gene trap lines (Daimon, Sato et al., in preparation). In the gene trap with GAL4/UAS system in B. mori, the gene trap vector can trap promoter activity and poly-A signal, efficiently.

  5. Future plan
  6. We are now studying enhancer trap and gene trap using other mutators, and want to incorporate the information to the database in future. Moreover, we plan to make a catalog of useful transgeninc strains such as other useful GAL4 and UAS strains (e.g. Tatematsu et al., 2009, Kobayashi et al., 2011, Sakurai et al., 2011). Also we will prepare the system of distribution of these resources for requests in near future by National Institute of Agrobiological Sciences and/or Kyushu University (Banno et al., 2010).

  7. References
  8. 1) The International Silkworm Genome Consortium (2008) The genome of a lepidopteran model insect, the silkworm Bombyx mori. Insect Biochem Mol Biol. Special issue on the Bombyx genome 38(12):1036-45
    2) Shimomura M, Minami H, Suetsugu Y, Ohyanagi H, Satoh C, Antonio B, Nagamura Y, Kadono-Okuda K, Kajiwara H, Sezutsu H, Nagaraju J, Goldsmith MR, Xia Q, Yamamoto K, Mita K (2009) KAIKObase: An integrated silkworm genome database and data mining tool. BMC Genomics 10(1):486
    3) Uchino K, Sezutsu H, Imamura M, Kobayashi I, Tatematsu KI, Iizuka T, Yonemura N, Mita K, Tamura T (2008) Construction of a piggyBac-based enhancer trap system for the analysis of gene function in silkworm Bombyx mori. Insect Biochem Mol Biol. Special issue on the Bombyx genome 38:1165-1173
    4) Sakudoh T, Sezutsu H, Nakashima T, Kobayashi I, Fujimoto H, Uchino K, Banno Y, Iwano H, Maekawa H, Tamura T, Kataoka H, Tsuchida K (2007) Carotenoid silk coloration is controlled by a carotenoid-binding protein, a product of the Yellow blood gene. Proc Natl Acad Sci U S A 104(21):8941-8946
    5) Ito K, Kidokoro K, Sezutsu H, Nohata J, Yamamoto K, Kobayashi I, Uchino K, Kalyebi A, Eguchi R, Hara W, Tamura T, Katsuma S, Shimada T, Mita K, Kadono-Okuda K (2008) Deletion of a gene encoding an amino acid transporter in the midgut membrane causes resistance to a Bombyx parvo-like virus. Proc Natl Acad Sci U S A 105(21):7523-7527
    6) Sezutsu H, Uchino K, Kobayashi I, Tatematsu K, Iizuka T, Yonemura N, Tamura T (2009) Conservation of fibroin gene promoter function between the domesticated silkworm Bombyx mori and the wild silkmoth Antheraea yamamai. Journal of Insect Biotechnology and Sericology 78(1):1-10
    7) Tatematsu KI, Kobayashi I, Uchino K, Sezutsu H, Iizuka T, Yonemura N, Tamura T (2010) Construction of a binary transgenic gene expression system for recombinant protein production in the middle silk gland of the silkworm Bombyx mori. Transgenic Research 19(3):473-87
    8) Kobayashi I, Kojima K, Uchino K, Sezutsu H, Iizuka T, Tatematsu KI, Yonemura N, Tanaka H, Ymakawa M, Ogura E, Kamachi Y, Tamura T (2011) An Efficient Binary System for Gene Expression in the Silkworm, Bombyx mori, Using GAL4 Variants. Archives of Insect Biochemistry & Physiology 76(4):195-210
    9) Sakurai T, Mitsuno H, Haupt SS, Uchino K, Yokohari F, Nishioka T, Kobayashi I, Sezutsu H, Tamura T, Kanzaki R (2011) A Single Sex Pheromone Receptor Determines Chemical Response Specificity of Sexual Behavior in the Silkmoth Bombyx mori. PLoS Genetics (in press)
    10) Banno Y, Shimada T, Kajiura Z, Sezutsu H (2010) The Silkworm-An Attractive BioResource Supplied by Japan. Experimental Animals 59(2):139-46

  9. Link
  10. KAIKObase: http://sgp.dna.affrc.go.jp/KAIKObase/
    SPG home page: http://sgp.dna.affrc.go.jp/
    Proteome database: http://kaiko2ddb.dna.affrc.go.jp/
    KAIKOGAAS: http://kaikogaas.dna.affrc.go.jp/
    KAIKOBLAST: http://kaikoblast.dna.affrc.go.jp/
    National BioResource Project Silkworm: http://silkworm.nbrp.jp/index_en.html
    Proteome database: http://kaiko2ddb.dna.affrc.go.jp/
    KAIKOGAAS: http://kaikogaas.dna.affrc.go.jp/
    KAIKOBLAST: http://kaikoblast.dna.affrc.go.jp/

  11. Contact us
  12. Hideki SEZUTSU, Ph. D.
    Transgenic Silkworm Research Unit
    National Institute of Agrobiological Sciences
    1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan
    tel/fax: +81-29(838)6091
    e-mail: bombyx-trap@nias.affrc.go.jp

Copyright (C) 2008 National Institute of Agrobiological Sciences. All rights reserved.